3-in-1 Screening Kit
How does it work?
The test kit is designed for the rapid detection of β-lactams, tetracyclines, and sulfonamide residues in milk samples at or above the established EU Maximum Residue Levels (MRL). It is suitable for the analysis of raw, pasteurised, and reconstituted milk powder used in dairy farms, milk collection centres, quality control laboratories, and dairy processing plants.
The kit supports compliance with regulatory limits established in the EU, India, and the USA.
This kit enables the simultaneous detection of multiple classes of antibiotics. The test utilises an immunochromatographic method based on a competitive antigen–antibody reaction.
When the test strip is immersed in the sample, free antibodies bind to the immobilised antigen on the test line. Antibodies that have reacted with antibiotic residues present in the sample do not bind to the test line and instead bind to the control line. The formation of coloured lines indicates the presence or absence of specific antibiotic residues in the sample.
In the box
Each box contains 96 test strips, allowing analysis of up to 96 samples.
Contents of the box:
- 96 test strips (6 plastic tubes; each tube contains 16 test strips and one 16-well reagent strip)
- 1 vial or plastic tube with an 8-well strip containing negative control
- 1 pack with an 8-well strip containing positive controls for tetracyclines, β-lactams, and sulfonamides
- 1 micropipette (200 µl) and 100 disposable tips
- 1 holding frame for wells
- User manual
Only one test strip is required per sample.
How to use it?
The test kit can be used with or without an incubator.
The test provides results within 4–5 minutes.
Detection limits of milk residue screening tests for beta-lactams, tetracyclines and sulfonamides
| β-lactams | MRL, µg/L | LOD, µg/L/l |
| Benzylpenicillin (Penicillin G) | 4 | 2-4 |
| Ampicillin | 4 | 3-4 |
| Amoxicillin | 4 | 4-5 |
| Oxacillin | 30 | 6-8 |
| Cloxacillin | 30 | 6-8 |
| Dicloxacillin | 30 | 6-8 |
| Nafcillin | 30 | 20-30 |
| Tetracyclines | MRL, µg/L | LOD, µg/L |
| Tetracycline | 100 | 100 |
| Oxytetracycline | 100 | 100 |
| Doxycycline | 100 | 100 |
| Chlortetracycline | 100 | 100 |
| Sulfonamides | MRL, µg/L | LOD, µg/L |
| Sulfametoxazole | 100 | 50 |
| Sulfadiazine | 100 | 100 |
| Sulfamethizole | 100 | 50 |
| Sulfadimethoxine | 100 | 100 |
| Sulfadimidine | 100 | 15 |
| Sulfachloropyrazine | 100 | 50 |
| Sulfisomidine | 100 | 30 |
| Sulfamonomethoxine | 100 | 30 |
| Sulfamethoxypyridazine | 100 | 60 |
| Sulfaquinoxaline | 100 | 100 |
| Sulfachlorpyridazine | 100 | 60 |
| Sulfamethoxydiazine | 100 | 40 |









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